The Casey Maguire Laboratory

For advanced therapeutic delivery

Technology I: exo-AAV.  exo-AAV are enveloped adeno-associated virus (AAV) vectors. In 2009, Casey Maguire and Johan Skog discovered and invented this technology and the Maguire lab has developed the platform ever since.  The envelope is derived from exosomes/extracellular vesicles which are  lipid based nanoparticles released by cells. Exosomes have several properties which may enhance AAV-mediated gene delivery.

**Example of a exo-AAV. Note double layer membrane of exosome and 5 AAV capsids inside. Cryo-EM performed by Professor Alain Brisson and Sisareuth Tan.**

Technology II: Adeno-associated virus capsid libraries and in vivo evolution is a powerful strategy to select vectors with robust gene delivery. We are using a novel AAV capsid library to select AAV vectors for a variety of organs and cells.

Efficient transgene delivery and expression (GFP) after an intravenous injection of a new AAV capsid, AAV-F, selected from our library. Collaboration with Dr. Eloise Hudry.

Efficient transduction of pericytes and smooth muscle cells in brain vasculature using a new AAV capsid, AAV-PR, engineered from a capsid library. Image courtesy of collaborator, Dr. Servio H. Ramirez

Transmission electron microscopic image of purified AAV9 vector from our lab.

Efficient gene expression (GFP) mediated by an AAV vector in sensory hair cells of the mouse inner ear. Hair cells are identified by myosin 7a staining (red). Image by Bence Gyorgy. Collaboration with David P. Corey, Harvard Medical School